DNA

Part:BBa_K4693006

Designed by: Vibhavari Medepalli   Group: iGEM23_IISER-TVM   (2023-10-10)


cDNA Serotonin 1

This is a short complementary DNA sequence that binds to the 44-mer serotonin aptamer (BBa_K4693000). Together with the aptamer, it forms an aptasensor that can quantify serotonin. Our team also designed a cDNA based on the inputs received from Dr. Tarun Kumar Sharma from Gujarat Biotechnology University, who suggested we design the cDNA to complement at least half the length of the aptamer, so this version of our cDNA 1 was 20 bp long with delta G value (-29.6 kcal/mol).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None